Proteomic Profiling of Secreted Proteins from CHO Cells Using Surface-Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry

摘要

Chinese hamster ovary (CHO) cells are the most commonly used host cell line for the production\udof recombinant biopharmaceuticals. These biopharmaceuticals are typically secreted from CHO\udcells and purified from harvested cell culture media. The purpose of this study was to investigate\udchanges in the secreted proteome of CHO cells over the various stages of the growth cycle\udusing Surface Enhanced Laser desorption ionization time-of-flight mass spectrometry (SELDITOF\udMS). Conditioned media samples were collected each day over a 6 day growth period\udfrom CHO-K1 cells grown in low serum (0.5% FBS) conditions in monolayer culture. Samples\udwere profiled on a number of ProteinChip arrays with different chromatographic surfaces. From\udthis study, 24 proteins were found to be differentially regulated at different phases of the growth\udcycle in CHO-K1 cells, when profiled on two chromatographic surfaces, Q10 (anionic) and\udIMAC30 (metal affinity) ProteinChip arrays.